I ordered latex beads, 1.0 um particle size both fluorescent and non fluorescent ( 1 mL suspension each) from sigma-Aldrich to use for neutrophil phagocytosis on flow cytometre, but dont undestand how to opsonise them. Hence, need simple description on how to opsonise these beads to allow neutrophils recognise and phagocytose them like bacteria.
You could try simply coating the beads with BSA (ie. incubate them 2h at RT with BSA in PBS, then centrifuge, resuspend in whatever medium you use to inject and sonicate them just before injection). Hope it helps!
Thanks a lot Maria, you've added more light. i'll resuspend in RPMI medium.
Hi Shaaya,
You can have a look to this paper (attached) from our group in addition to Awatif's suggestion. The study is with macrophages and dendritic cells, though you can have some information on how to coat beads.
Good luck,
Andres
Hello Andres,
What a nice and interesting article for my quest! Thank you so much.
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