Dear Shiva,

The following book may be helpful:

Modern Alkaloids: Structure, Isolation, Synthesis and Biology

http://onlinelibrary.wiley.com/doi/10.1002/9783527621071.ch13/summary

Hoping this will be helpful,

Rafik

Hello

You can refer Thin layer chromatography by Wagner and Bladt to determine it by TLC or HPTLC

or else you can refer Phytochemical methods by Harborne.

 Alkaloid determination using Harborne (1973)

method:

5 g of the sample was weighed into a 250 ml

beaker and 200 ml of 10% acetic acid in ethanol

was added and covered and allowed to stand for 4

h. This was filtered and the extract was

concentrated on a water bath to one-quarter of the

original volume. Concentrated ammonium

hydroxide was added dropwise to the extract until

the precipitation was complete. The whole solution

was allowed to settle and the precipitated was

collected and washed with dilute ammonium

hydroxide and then filtered. The residue is the

alkaloid, which was dried and weighed

.

Thai J. Pharm. Sci. 32 (2008) 17-20 17

Short report

Spectrophotometric determination of total alkaloids in some Iranian medicinal plants

this reference is available in internet

Dear collegue there is many research papers and works about quantitative estimation of alkaloids , but there is no books try this method from research work of Manjunath Ajanal, et al

if you need more information please ask me 

Extraction

The 100 gm of each plant material was ground and then extracted with methanol for 24 hours in a continuous extraction (Soxhlet) apparatus. The extract was filtered and methanol was evaporated on a rotary evaporator under vacuum at a temperature of 45°C to dryness.

Qualitative estimation (Test for alkaloids)

Presence of alkaloid was confirmed by Dragendroff's method.[15] A part of extract was dissolved in dilute HCL and 2 drops of Dragon drop's was added, a crystalline precipitate indicates presence of alkaloid. The sample which showed positive alkaloid was then subjected to further quantitative evaluation.

Preparation of reagents

Bromocresol green solution was prepared by heating 69.8 mg bromocresol green with 3 ml of 2N NaOH and 5 ml distilled water until completely dissolved and the solution was diluted to 1000 ml with distilled water.

Phosphate buffer solution (pH 4.7) was prepared by adjusting the pH of 2M sodium phosphate (71.6 gm Na2HPO4 in 1 L distilled water) to 4.7 with 0.2 M citric acid (42.02 gm citric acid in 1 L distilled water).

Atropine standard solution was made by dissolving 1 mg of pure Atropine (AR-grade procured from Sigma Company) in 10 ml distilled water.

Seperation of Alkaloid

A part of extract residue was dissolved in 2N HCL and then filtered. 1 ml of this solution was transferred to separatory funnel and washed with 10 ml chloroform (3 times). The pH of this solution was adjusted to neutral with 0.1 N NaOH. Then 5 ml of BCG solution and 5 ml of phosphate buffer were added to this solution. The mixture was shaken and complex extracted with 1, 2, 3 and 4 ml chloroform by vigorous shaking, the extract was then collected in a 10 ml volumetric flask and diluted with chloroform.

Preparation of standard curve

Accurately measured aliquots (0.4, 0.6, 0.8, 1 and 1.2 ml) of Atropine standard solution was transferred to different separatory funnels. Then 5 ml of pH 4.7 phosphate buffer and 5 ml of BCG solution was taken and the mixture was shaken with extract with 1, 2, 3, and 4 ml of chloroform. The extracts were then collected in 10 ml volumetric flask and then diluted to adjust solution with chloroform.

The absorbance of the complex in chloroform was measured at spectrum of 470 nm in UV-Spectrophotometer (SHIMADZU UV-1800) against the blank prepared as above but without Atropine.

You can refer " Phytochemical Methods - A guide to Modern Techniques of Plant Analysis" Authored by J. B. Harborne.

 You may find the following links of websites and names of the books helpful 

http://www.worldcat.org/title/studies-on-the-quantitative-estimation-of-alkaloids-by-means-of-immiscible-solvents/oclc/435687832

Quantitative determination of alkaloids in belladonna tincture USP. Gupta VD.

Modern Methods of Plant Analysis

There are many books available on kindle also.