Hello! I am currently writing my first paper to be published, and I would love some advice on how to explain the statistical analysis of my experiment. For my study I grew bacteria in two cell lines, with and without cycloheximide (4 treatment groups total). I then harvested the flasks for 14 days and quantified the bacteria in my samples. I grew each flask in duplicate and tested them in triplicate, so I ended up with 6 data points per treatment group per day.
I averaged these data points to create a line graph to visualize growth, and I used excel to perform ANOVA using 2 treatment groups and 1 harvest day at a time, to see if they were significantly different on each day. I need to briefly describe this in my methods section, but I am having trouble wording it in a professional way, as it was a pretty simple process.
Any advice would be appreciated! Thanks!
Alex H
CDC ORISE Fellow
I would define your design a Between x Between x Within factorial repeated measures design. "Factorial" because the 2 between factors together with the within factor all are independent of each other.
See: https://en.wikipedia.org/wiki/Factorial_experiment
Between subjects factor 1: 2 Cell lines
Between subjects factor 2: 2 Cycloheximide treatments (with or without = placebo)
Within subjects factor : 14 days of observations
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