Hello! It looks more like Stanieria that is producing high amounts of Phycoerythrin but I would recommend you to amplify the partial 16S rRNA gene of this cyano with primers of Nubel: PCR primers to amplify 16S rRNA genes from cyanobacteria.

U Nübel, F Garcia-Pichel and G Muyzer.

I also had some difficult strains of cyanobacteria and these primers helped alot.

Also compare your micrographs with species given in http://www.cyanodb.cz/.

The protocols described in these articles may help you with molecular part of your research:

More´, M.I., Herrick, J.B., Silva, M.C., Ghiorse, W.C. and

Madsen, E.L. (1994) Quantitative cell lysis of indigenous microorganisms

and rapid extraction of microbial DNA from sediment.

Appl. Environ. Microbiol. 60, 1572–1580.

[20] Nu¨ bel, U., Garcia-Pichel, F. and Muyzer, G. (1997) Primers to

amplify 16S rRNA genes from cyanobacteria. Appl. Environ.

Microbiol. 63, 3327–3332.

Good luck!

If you want to have a best DNA extraction from cyanobacterial strains, you will use gram-positive bacteria DNA extraction protocol.

Thanks Nübel primers actually confirmed Stanieria

Thats great!!!

Hello just to clarify... Thanks to a recent work (Shalgyn et al. 2019) and that we sequenced completely 16S (27F 1492R primers)we have reclasified the culture as Chroococcidium gelatinsum, get ready for we are publishing this year and depositing our culutre in NIES collection

Greetings!!

Sergei Shalygin

In this case the closest sequence relative would be Foliisarcina, altough first descriptions of these cyano were made in Alchichica lake with Tavera & Komarék 1998 as Chroococcidium gelatinosum.

We think its necesary to establish this genera because it belongs to the Xenococcacae family for shure. Anyway we are sending this culture along others to the NIES collection next monday, If you are interested I can share you my 16S sequences and tell me your opnion. Best regards!

Sergei Shalygin