Mysterious line across SDS gel?

Does anyone have the similar issue? A line across the denature SDS gel shows up after staining with Coomassie blue and there are many lines in every lane if you take a closer look. This gel had 15...

07 January 2021 5,610 4 View

Look for Book?

Can anybody give me the following writer book? Helen Deresky "International Management"

26 December 2020 5,109 5 View

Dose the metals oxides like (Fe3O4, ZnO TiO2) can coordinate with organic linker in the purpose of MOFs synthesis?

Dose the metals oxides like (Fe3O4, ZnO TiO2) can coordinate with organic linker in the purpose of MOFs synthesis?

09 December 2020 4,896 2 View

What statistical method to use to identify potential confounders in a large set of explanatory variables?

I am working on identifying biomarkers of the outcome of treatment in a disease. I have 20 potential biomarkers and more than 30 potential confounders. Actually, I know that some of those...

11 November 2020 3,794 3 View

How can I make my Caki-2 cell line grow?

Hello everyone! I have trouble with a cell line, namely the human kidney carcinoma Caki-2 cell line. Another lab sent us a few criovials of cells, with their growing conditions, but I can't grow...

24 October 2020 2,568 3 View

Look for details for the following reference for citation?

22 October 2020 9,252 4 View

Look for methodology?

Evaluating training design is similar to evaluating trainees reaction?

19 October 2020 4,575 4 View

Look for methodology?

I will do single group pre post test in evaluating training. In that case, what strategy i should follow between experimental or survey research?

03 October 2020 5,326 6 View

Efficiency of four fed patch antenna?

Hello, there is four fed patch for best circular pol simulated in CST. When 1 port is excited the S31 is about -4dB, S21=S41=-13dB, S11=-15dB. It means that total efficiency of antenna is less...

20 September 2020 1,725 3 View

Can anybody give me the reference including page no of the following citation?

Mayer and Pipe (1983) suggests that the reasons for strategic plan for training evaluation is to evolve a careful methods of assessing and reporting training effectiveness, so that the...

19 September 2020 2,319 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

How would I use a glycerol fixation to fix whole brain Drosophila tissue?

I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.

21 February 2021 3,081 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Is there any general protocol for immunohistology staining?

I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?

17 February 2021 3,062 4 View