I am trying to investigate the antibacterial effect of Postbiotic on S.mutans. However, because of the slow growth of mutans on Muller Hinton agar plates, I am unable to clearly observe the test results within 24 hours. I am looking for a suitable protocol to carry out this test. If anyone has information regarding the challenges of working with the Streptococcus mutans, I would greatly appreciate your help.
Yes, it is possible to use Brain Heart Infusion (BHI) or Tryptic Soy Broth (TSB) instead of Mueller-Hinton agar for the agar well diffusion test with Streptococcus mutans. These media are more suitable for S. mutans growth and can provide clearer results within 24 hours.
When conducting the agar well diffusion test:
By using these growth-promoting media and optimized conditions, you should be able to obtain clearer results for your antibacterial tests with S. mutans within a 24-48 hour timeframe.
Yes, it is possible to use Brain Heart Infusion (BHI) or Tryptic Soy Broth (TSB) instead of Mueller-Hinton agar (MHA) in the agar well diffusion test for Streptococcus mutans, but the choice of medium can influence the results. Here are the factors to consider
BHI and TSB: These are nutrient-rich media that support fastidious organisms like Streptococcus mutans. However, their rich nutrient composition may interfere with the diffusion of certain antimicrobials or create overly dense bacterial growth, which could skew the results.or accurate and reproducible results
If you proceed with BHI or TSB, ensure the methodology and limitations are clearly stated, and consider comparing results with MHA in parallel for validation
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