i want a protocol for coating of non-biotyne labeling
and shows no error or explanation about the reason .any one can help me?I think I am doing each step correctly
20 June 2024 3,295 2 View
I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...
10 June 2024 9,564 6 View
I have 7 peptides with 7 or more amino acids and I want to know that the peptides must be with biotin.
23 January 2024 5,185 0 View
The Concise Version of IRAN Handicrafts and Traditional Arts was provided and the Comprehensive version will be released soon in collaboration with Dr. Senapathy Marisennayya. We also welcome any...
03 September 2023 5,914 4 View
I am learning the pymc package from the book "Bayesian Methods for Hackers: Probabilistic Programming and Bayesian Inference". While executing some codes shown in the picture, I got errors. Does...
07 March 2023 2,216 1 View
Dears I want to interfere the autophagy pathway in osteoclasts using 3-MA and rapamycin. In the literature I found some controversial information about the concentrations. I would ask if...
29 June 2022 7,879 0 View
I check the viability of breast cancer cell lines(MDA-MB-231 and MCF7) with 10-OH-Camptothecin (CPT) to get the effective concentration of this drug. I used RPMI 1640 for cell culture (our lab did...
15 August 2021 2,688 0 View
Ancova repeated measures or manova rm.my participants receive one of the interventions.my other question is if the multivariate tests table in anova rm did not show any significant value but...
30 April 2021 2,581 4 View
we have some LG membranes that have gained too much weight (20-24 Kg). Can we lower their weight through harsh cleaning, and if so what are the appropriate temperature, pH and chemicals that we...
25 April 2021 8,586 1 View
Hi, I try to simulate a graphene-based structure. At the beginning of the simulation, the auto-shutoff level decreases, but, approaching the end, the auto-shutoff level increases, while the...
16 April 2020 4,992 3 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...
23 July 2024 7,479 3 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
