To measure FAMEs in vegetable oils using your Shimadzu GC-2010 PLUS, first transesterify the oil with methanol and a catalyst, then analyze the extracted FAMEs on a polar capillary GC column with FID detection. Use a standard FAME mix for peak identification and quantification under a temperature-programmed GC method.

refer AOAC

You can check with FAME method by Metcalfe, Smith and Pelca 1966. In this method you can analyse any vegetable or other oils FA composition. First you check with Std individual FAs and then Std FAME mix identify the RTs. Then inject unknown FAs for your quantification.

Thanks,