How can I validate the UV spectroscopy?

can I compare UV spectroscopy reading with nanodrop reading to validate the UV spectroscopy? or is there a better way to know if the UV works well or not? Note: we use it in DNA, RNA &...

12 December 2020 4,869 2 View

What's the right way to Tween 20 storage?

02 December 2020 9,955 2 View

I need strains of Agrobacterium rhizogenes or Agrobacterium tumefaciens, how can I get them?

I need the above strains for my students .

02 October 2020 5,867 5 View

What's makes RNA (and not DNA) bind to the silica spin column in commercial kits?

In silica spin column-based kits, if the spin column material is the same and the binding to the column occurs by using ethanol and Guanidine salts. Then, what's the difference between the RNA...

20 September 2020 4,798 1 View

What is the top ten scientific journals in the world, and how quality is evaluated?

Do quality is evaluated just depends on the impact factor (IF)? what is the greatest number of the IF?

18 July 2020 6,135 44 View

What is the main reason of global warming? Do CO2 is responsible only? Do wildfire an auto event?

The average temperature of the Earth is rising at nearly twice the rate it was 50 years ago. This rapid warming trend cannot be explained by natural cycles alone, scientists have concluded. The...

08 June 2020 3,764 38 View

Why Non specific bands of PCR reaction appear while using specific primers for tobamoviruses?

i have done RNA extraction and cDNA for tobamovirus then i made a PCR reaction using primer pair Tob uni1/Tob uni2 according to the protocol in Letschert et al., 2002 ( annealing temp. 60C for 45s...

09 December 2019 7,276 3 View

Who can help me by giving some lectures about Agriculture projects?

I really need to some lectures are relationship to agriculture projects, it will be by PPT, PDF and any thing.

03 October 2019 766 5 View

How to get data from a tree node for wavelet package in matlab?

I use the function (wpdec2) in wavelet packet

28 April 2019 1,672 2 View

Do I have the appropriate sonicator?

I am following the catalyst preparation procedure expressed in the attached paper [Applied Catalysis B: Environmental 239 (2018) 280-289]. The procedure requires sonicating

14 October 2018 8,513 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How to improve the adhesion of epoxy coating on steel substrate? Which factor affects?

I am facing a problem regarding a de-adhesion problem with the coating. A brief description of my methodology is given below. I am using mild or Low Carbon steel as a substrate and I polish the...

14 February 2021 2,167 4 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

How long can synthesized peptides using SPPS be stored? Storage Conditions?

I have been synthesizing peptides on resin beads. I am wondering what best storage method is there to store the beads after synthesis before cleaving. And how long can they be stored in that...

02 February 2021 5,636 5 View

The peptide synthesized with CTC resin have mass more (+68 Da) than the desired mass of the synthesized peptide. What could be the reason?

I am synthesizing 7 amino acid long peptide sequence with CTC resin, 50oC coupling for 10 min. On cleavage (TFA/TIPS/Water 95:2.5:2.5) I got peptide with good yield. However, It has 68 Da more...

30 January 2021 8,483 3 View

Which method is preferable for cutting the nanocomposites?

Which of the methods like water jet cutting, diamond cutting, laser cutting, etc. is more suitable to cut the carbon nanotubes reinforced epoxy composites into test sample to ensure the clean...

29 January 2021 1,354 5 View