Lignocelluloses degrading enzyme (CMCase, β-glucosidase, and Xylase) important for utilization agriculture crop residue and fast growing biomass for energy production, which one needful for next generation, because in case of Jatropha curcus are not able to achieve target 2030 as biofuel (currently bio fuel is about 2% of total gasoline consumption in the world, which could reach 10–20% by 2030)