Currently I measure Na+ with the ratiometric dy SBFI-AM in Acute Brain (P16 Mouse Slice). I use a Lmbda DG-1 for Exitation but i have high nois in my measurments. How can i reduce that. ? Some good ideas or need more informations just ask. ;-)
Dear Jan, what was the sampling rate of your data. We can use an appropriate filter for this signal. Information about IIR filter and FIR filter...https://www.minidsp.com/applications/dsp-basics/fir-vs-iir-filtering
What sort of detector are you using? Camera? PMT? Photodiode? What frequency of noise? Low? High? 60-cycle? Is the noise in the measuring device, or variation in the light source? The answer depends very much on the details. When you say "noisy" is it just that you need to average a lot? I haven't used it, but my impression is that SBFI shows weak percent changes in fluorescence, so many repetitions may be necessary.
I use an Camere Orca Flash i measure by 1 HZ. I think it is beause of the intensety of the wavelenght. 340 is by 4000+- and 380 by 8000+-. I guess that causes the noise in the ratio but i dont know how to miniemise this intensety difference.
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