Dear S.Rasoul Hashemi,

The following publications describe the variety of methods used to recover natural products from plants and animals:

1-Isolation of Natural Products

*Sharad Visht, Swati Chaturvedi.

Current Pharma Research

ISSN: 2230-7842

CPR 2(3), 2012, 584-599.

Review Article

Abstract

Plant had been used as medicine in ancient. Now day’s Pharmaceutical companies start processing of medicinal and aromatic plants in their formulation by using extraction of active components. Extraction of plant components likes volatile, Essential or ethereal oils and mixtures composed of volatile liquid and solid

compounds depend upon their composition and their boiling point. Now days there are several processes like distillation, enfleurage, maceration, expression, solvent extraction and fluid extraction are available for extraction of plant component. This review also summarizes the characters of phytoconstituents, choice of solvents, influence of solvents, extraction strategy, procedures for extraction of herbal drugs and treatment of drug residue after extraction. 

https://encrypted.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&uact=8&ved=0ahUKEwiqsfTCzN_MAhXJ8RQKHRZLB1EQIAgrMAE&url=https%3A%2F%2Fwebcache.googleusercontent.com%2Fsearch%3Fq%3Dcache%3A_RmDZY4eI_kJ%3Ahttps%3A%2F%2Fwww.researchgate.net%2Ffile.PostFileLoader.html%253Fid%253D55dd2b656225ff899d8b45a4%2526assetKey%253DAS%25253A273838879772676%2525401442299685123%2B%26cd%3D2%26hl%3Den%26ct%3Dclnk&usg=AFQjCNE85UHl5ST3YOPmQD3vSdlf7zmasw&sig2=2X4IDtaZdVwqH9vWmM3OoA

2-Journal of Chemical and Pharmaceutical Research, 2014, 6(1):338-345

Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5

338

The extraction, separation and purification of alkaloids in the natural medicine

JI Yubin1,2* , Yu Miao1,2 , Wang Bing3 and Zhang Yao1

1Center of Life Science and Environmental Science, Harbin University of Commerce, Harbin China

2Engineering Research Center of Natural Anti-tumor Drugs, Ministry of Education, Harbin China

3School of Food Engineering, Harbin University of Commerce, Harbin, China

_____________________________________________________________________________________________ ABSTRACT

In this paper, I will have natural medicine alkaloids extraction, separation and purification methods for a talk, the presence of alkaloids of natural medicine are in the form of free or salt alkaloids, so inorganic acidic water can be used to extract in order to have alkaloids organic acids replaced with inorganic acid salt and increase its solubility; The free alkaloids or salt both can be dissolved in methanol, ethanol, we can use heated alcohol under reflux extraction or ultrasonic alcohol extraction; and most of the free alkaloid is lipophilic, also can use heated alcohol under reflux to extract free alkaloids with the organic solvent such as chloroform, benzene, ether and so on. Most obtained alkaloids by extracting are mixture, according to the alkaloids class, basicity, solubility differences and the special functional group of alkaloids to separate the alkaloid mixture. Then the separated monomer detectable.

https://encrypted.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwiqsfTCzN_MAhXJ8RQKHRZLB1EQIAgkMAA&url=https%3A%2F%2Fwebcache.googleusercontent.com%2Fsearch%3Fq%3Dcache%3AVaGBdGd6aXEJ%3Ahttps%3A%2F%2Fwww.researchgate.net%2Ffile.PostFileLoader.html%253Fid%253D567ca7dd5dbbbdb22e8b4567%2526assetKey%253DAS%25253A310372647538688%2525401451010013156%2B%26cd%3D1%26hl%3Den%26ct%3Dclnk&usg=AFQjCNG8tFvDUh9xHaqMEnX8qr74xDmnSA&sig2=WjT3fwmkm5XEHDcaw-Ehuw

3-Methods Mol Biol. 2014;1153:171-85. doi: 10.1007/978-1-4939-0606-2_12.

Analysis of steroidal alkaloids and saponins in Solanaceae plant extracts using UPLC-qTOF mass spectrometry.

Heinig U1, Aharoni A.

Abstract

Plants of the Solanaceae family are renowned for the production of cholesterol-derived steroidal glycosides, including the nitrogen containing glycoalkaloids and steroidal saponins. In this chapter we describe the use of UPLC (Ultra Performance Liquid Chromatography) coupled with qTOF (Quadrupole Time-of-Flight) mass spectrometry for profiling of these two large classes of semipolar metabolites. The presented method includes an optimized sample preparation protocol, a procedure for high resolution chromatographic separation and metabolite detection using the TOF mass spectrometer which provides high resolution and mass accuracy. A detailed description for non-targeted data analysis and a strategy for putative identification of steroidal glycosides from complex extracts based on interpretation of mass fragmentation patterns is also provided. The described methodology allows profiling and putative identification of multiple steroidal glycoside compounds from the assortment of Solanaceae species producing these molecules.

http://www.ncbi.nlm.nih.gov/pubmed/24777797

Hoping this will be helpful,

Rafik

Dear Rafik karaman

Thanks for your reply.

I've added alkaloid to the oil and now I want to separate it from the oil

Dear Rasoul,

The following publication demonstrates an extraction method of solasodine which should be applicable in your case:

Extraction of Solasodine from Dry Fruits and Leaves of Solanum

laciniatum Ait. and the Synthesis of 16-Dehydropregnenolone Acetate

from Solasodine by Phase-Transfer Catalysis

Abstract

A simple extraction and isolation process of solasodine, a natural precursor to synthesize steroidal drugs, from fruits and leaves of Solanum laciniatum Ait. was developed. The optimum concentration of 2-propanol for the extraction of crude glycosides was 70%. The suitable hydrolysis condition of solasodine from crude glycosides was by 1 N hydrochloric acid in 2-propanol. Pure solasodine from both fruits and leaves of Solanum laciniatum Ait. was obtained without any requirement of column chromatography. The yield of pure solasodine were 0.34±0.04% and 0.44±0.16% of the dry weight of fruits and leaves, respectively. The maximum yield of 37.0% of pure 16-DPA was obtained by using tetrabutylammonium hydrogen sulfate as a phase-transfer catalyst and potassium dichromate as an oxidizing agent. The results indicated the novel economic with environmental friendly method of solasodine extraction and synthesis of 16-dehydropregnenolone acetate from solasodine by phase-transfer catalysis.

http://wwwlib.teiep.gr/images/stories/acta/Acta%20679/679_12.pdf

Hoping this will be helpful,

Rafik

Dear Rafik karaman

Thank you very much