Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

How do you calculate a discrimination index for polytomous data?

Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...

13 February 2021 3,517 2 View

Regarding immunology, transmissivity, herd immunity and vaccines in Sars CoV-2?

This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....

08 February 2021 8,321 3 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Can I polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

I want to perform the synthesis of a hydrogel and I want to know if I can polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

02 February 2021 2,106 6 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT?

Im looking for any papers, reports,articles, links etc on information on How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT.

24 February 2021 2,750 4 View

Do the introduced salts (NaOH/ HCl) influence the spectroscopic shifts of the fluorescence excitation-emission matrix?

During the resin (XAD-8) fractionation of wastewater salts (NaOH or HaCl) are used for acid or base elutions/pH adjustments. Since the amount of introduced salt is minimal, so is it required to...

08 February 2021 9,489 2 View

What is the current situation in the development of nanofibers laminated and biodegradable material based facemasks?

I want to know the progress in the development of polymer laminated, biodegradable material based facemasks.

18 January 2021 9,205 3 View

Why does COD increase over time when external carbon sources are added in the denitrification process in waste water?

Why does COD increase over time when external carbon sources are added in the denitrification process in waste water? After 1 hour, the concentration increased from 30mg/l to 40mg/l after 20...

17 January 2021 374 9 View

Seperate of organic and inorganic phosphorus in waste water?

Is it possible to separate organic and inorganic phosphorus in wastewater with a filter?

14 January 2021 952 3 View

Why wont my microbial fuel cell generate any current?

I have constructed a 2 chamber MFC for my senior research assignment and it has yet to produce any voltage. I used nafion as a PEM membrane and used graphite brushes as the electrodes. I have the...

11 January 2021 5,553 4 View

Response Surface Methodology?

I'm using 3 different light intensities (constant values 15,30,45 W) for the wastewater treatment of an organic compound and optimizing the parameters (pH, catalyst dose and light intensity)...

31 December 2020 2,377 4 View

How can Meta-Analysis be applied to the Life cycle assessment (LCA) of Wastewater treatment Plants (WWTPs)?

I am new into the meta-analysis concept. Having done a little reading and watched videos on YouTube, I understand there are different kinds of meta-analysis. And the type of summary statistics or...

29 December 2020 3,677 3 View

I would like to know any methods and steps could be use to treat waste containing mixture of alcohol solvent, oils and glycerol?

I'm not sure the steps to treat wastewater containing mixture of alcohol solvent, oils and glycerol. do we neet to separate before further treatment.

27 December 2020 6,058 6 View