I am trying to quantify sterols just up-stream and down-stream the Cycloartenol in Rice. Do you know which ones are the most abundant ones in Rice? and which ones are the most easily detectable?
How can we estimate sample size for a moderated mediation analysis with more than two mediators? I am looking for scripts, macros, syntaxes, that allow us to estimate sample sizes for research...
15 February 2021 6,813 3 View
How effective and helpful is it, really.
25 January 2021 6,988 73 View
Hi! I am having problems cloning a 1.6 kb fragment containing 2 different genes and 2X2A peptides into a vector that I already used to clone other fragments (digested with PmeI/BamHI). The vector...
17 December 2020 5,218 3 View
What are the possible topic in this time except pandemic? Thank you in advance for the suggestion.
06 December 2020 5,380 7 View
We are trying to find alternatives to the use of fluorescent tagged primers and capillary electrophoresis to access somatic activity of the target gene. Thank you!
25 September 2020 4,761 3 View
I have a question for KLH experts. Is possible that an antibody drug conjugated (ADC) against KLH-fluorquinolone has a strong effect in T cells viability? Is a T- cell expressing KHL- or...
24 September 2020 2,931 1 View
Hello, I ma an undergraduate student studying environmental science and about to start on my dissertation and I am still thinking around a topic.I am interested in technology and sustainability...
24 August 2020 3,930 11 View
I need to perform a meta-analysis of 2 GWAS studies and I need assistance with the input study files (plink --assoc.logistic) as well as the output meta-analysis file (.TBL). - Firstly, I am not...
07 June 2020 5,831 1 View
I don't seem to find anything published (or unpublished) to answer my research question. Broadly, when I search for "PTSD + Virtual Reality + First Responders" (and all the variants these words...
11 March 2020 3,349 7 View
I don't understand why we use HPLC as method to determine the purity of NCE/drugs in medicinal chemistry if the HPLC won't show everything that could be a impurity (only compounds with UV absortion).
02 February 2020 3,135 3 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...
27 February 2021 8,128 1 View
Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...
26 February 2021 6,253 3 View
I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...
26 February 2021 1,511 3 View
Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...
26 February 2021 1,100 5 View
Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...
25 February 2021 9,602 4 View
Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...
24 February 2021 2,602 1 View