I need a protocol for extracting ochratoxin A from green coffee beans for analysis on hplc, as well as the protocol for carrying out the calibr ?

ochratoxine A ; HPLC ; green coffee

29 December 2020 4,028 3 View

Could anybody give me information about room temperature degradation of silicone oil to silicon oxide?

Does anyone have any experience of room temperature degradation of silicone oil to silica in presence of acids?

26 December 2020 1,275 4 View

Should i add ATG in the beginning of my polypetide sequence when i want to insert it in a phagemid?

Hi I want to make a recombinant m13 phage displaying my peptide as a fusion peptide on its surface pVIII. Should i add ATG at the beginning when i want to insert it in phagmid vector? Previously,...

18 December 2020 9,931 2 View

What is the most recommended multispectral-UAV to be used for delineating the shoreline?

Hi there, I am looking to purchase an UAV with an attached multi-spectral camera to use its images in delineate the shoreline. I need to take a vertical geo-referenced image (if possible) and...

21 November 2020 1,283 3 View

How can i delaying a signal in Matlab script file without using simulink block (i want the instruction of delaying a signal in script file ) ?

How can i delaying a signal in Matlab script file without using simulink block? (i want the instruction of delaying a signal in script file )

27 October 2020 4,299 2 View

What would you do if MgSO4 did not fully dry your organic extract ?

Hi everyone, I used MgSO4 as a drying agent to suck all water droplets from my organic extract. However, even after adding MgSO4 three times and leaving it overnight, I still see some water...

14 September 2020 9,203 3 View

What multiphase model should be used to simulate the evaporation of an oil droplet in ANSYS FLUENT?

Hello, we have a sheet of Aluminium and some droplets of oil on it. The temperature of the sheet increases and the oil droplet evaporates in the air. The droplet has water and kerosene too and...

29 August 2020 2,268 3 View

How can I download Haiti earthquake datasets?

How can I download Haiti 2010 GeoEye Satellite images or any other datasets regarding this mentioned study area? Is there any FTP server or website to access? Or If anyone has this data set, I'm...

07 August 2020 4,872 3 View

How to activate the solid species tab in species transport in ANSYS Fluent?

Hello, I have to add a solid catalyst to the mixture but the tab is not activated. I have defined the solid material in the materials section but the list is showing the fluid materials only. How...

19 July 2020 4,930 3 View

How to make intervals in an ANSYS Fluent user defined function UDF to describe three different equations through time?

Hello, I have a plot shows the change of a quantity with time. How can we write a code in Fluent UDF to compile it ? I need a way to define the time intervals. From start to a specific moment, the...

30 May 2020 5,774 10 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

Hi, can a variable in a questionnaire be measured with a series of questions or it has to one question for one variable?

I am doing research on the impact of competitive bidding on project success and would like to use SEM. Project success shall be: cost overage, completion time, quality of works and variation...

13 February 2021 4,413 6 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

What is your opinion regarding opening of school? Now students are supposed to join school. Do you think that decision is in the interest of society ?

From long Pendamic now government is opening school and colleges on for off line classes. What do you think that this decision is fruitful to the students and society too?

08 February 2021 3,068 6 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View

Looking for a scale to measure attitudes towards violence against women and the factors that impact attitudes in South Africa?

I am interested in doing research on attitudes towards violence against women and the factors that impact attitudes in South Africa? Does anyone know which attitude scale I can use to measure...

06 February 2021 3,076 1 View