These bacteria can be found in malolactic fermentation and are used to induce malolactic fermentation in wine. We already have these bacteria isolated, but now we're going to grow them on an Erlenmeyer flask and then in a bioreactor of 1 liter volume.
There's a little problem, I think it's hard to start the growth in the flask and then in the bioreactor. The conditions are different and it seems these bacteria are a bit more delicate than we thought. So, is there any protocol to start growing these bacteria from laboratory scale to industrial scale?
Dear Miguel, O.oeni is indeed a very sensitive LAB with high auxotrophic needs. Our Biotechnology group in Swansea University, has done extensive research on LAB in particular Dr. Robert W. Lovitt and Dr. Ilseon Jung have employed O.oeni for malolactic fermentations. I am attaching here the two links and info of their published papers I hope this helps, http://onlinelibrary.wiley.com/doi/10.1002/jctb.2424/abstract A comparative study of the growth of lactic acid bacteria in a pilot scale membrane bioreactor Ilseon Jung*,Robert W. Lovitt and A comparative study of an intensive malolactic transformation of cider using Lactobacillus brevis and Oenococcus oeni in a membrane bioreactor. Jung IS, Lovitt RW. http://link.springer.com/article/10.1007%2Fs10295-010-0716-7 .
Best wishes, Myrto
Yes we isolated O.oeni in cassava dought (mainly use as alcoholic beverage) and we used the a liquid media based on MRS in wich glucose were replace by précooked cassava starch. This was done in a 2liter biolafite reactor (for stater production) then in 7liter and 50l reactors for cassava based vine second fermentation (after alcohlic fermentation by Rhyzopus orizae). These data are under publication but similar work were done in the same media with Lactobacillus plantarum strains u can have a look at the paper here http://www.ajol.info/index.php/cajeb/article/view/37937
cheers
Thanks both of you guys, I'm still doing this pre-project in my college and this topic is pretty interesting, I think we won't use MBR, just a BIOSTAT® Aplus bioreactor. I'm still curious about this bacteria growing.
I'll keep you informed!
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