Is there any thiol-gold bond reducing agent that won't interfered with the Ribogreen assay?
Hi,
These are new publications on the topic:
Achieving long-term stability of lipid nanoparticles: examining the effect of pH, temperature, and lyophilization
RL Ball, P Bajaj, KA Whitehead - International Journal of …, 2017 - ncbi.nlm.nih.gov
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Aptamer-functionalized hybrid nanoparticle for the treatment of breast cancer
D Powell, S Chandra, K Dodson, F Shaheen… - European Journal of …, 2017 - Elsevier
... Fig. 3. Determination of siRNA's encapsulation efficiency of the selected nanoparticles. The entrapped siRNA in three formulations (ie F21, F31 and F40) was decomplexed by exposure to 1% SDS for 18 h and then measured by Ribogreen Assay. ...
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Improving the osteogenesis of rat mesenchymal stem cells by chitosan-based-microRNA nanoparticles
G Wu, C Feng, G Hui, Z Wang, J Tan, L Luo, P Xue… - Carbohydrate …, 2016 - Elsevier
... Nanoparticles were formed instantaneously upon the drop-wise addition of TPP/HA solution to
a fixed volume of chitosan solution under ... were centrifuged at 12,000 rpm for 30 min and the
antimiR-138 in the supernatant was quantified using RiboGreen ® assay according to the ...
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Thanks for this! I actually ended up using DTT to strip particles and used Ribogreen - there was no background from DT after all
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