Has anyone standadized the extract when phytoconstituent is not known?
Also a qualitative phytochemical screening using various standard methods will afford you to know the phytoconstituents that are in your plant extract.
HPLC could be another answer, I think... especially in case that you can be aided by LC-MS.
Just see fingerprinting (chemical and DNA) .
Personally I would suggestyou to go for NMR spectra for standardization of the extract. You need not to isolate constituents for stadardizing the extract. There are a number of parameters you can ponder upon.
wbw
rks
Dear Dr. Devang J. Pandya
Without knowing marker compound how the HPTLC fingerprint help to standardise plant extract. I think that if you analyse by HPTLC or HPLC fingerprints of extract with any marker then only you can say that your extract is standardized and the percentage of marker can be used by future researchers in standardization of the same plant extract.
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