Has anyone performed aromatase inhibition assay using HPLC-UV to detect estrone?

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Bruce Neagle

As I recall Estrone is quantified in a LC-UV using 50/50 Methanol/Water mobile phase.

Karthikeyan Periyasamy

please try this http://www.phenomenex.com/Application/Detail/19680

Klaus Belsner

I've done estrogen it in female urine/blood in 1995, with a buffer nearly equal to the one described by http://www.phenomenex.com/Application/Detail/19680 on Hypersil ODS (150x2mm), however detection was performed with an electrochemical detector (glassy carbon electrode). The electrochemical detector offers a lot of selectivity.