Do you need to acidify your samples prior to desalting with C18?

I am wondering how important pH is here. I have a sample tryptic sample in 50mM TEAB, wondering if I need to add in TFA to ~2% before desalting. I understand the need to acidify samples with ion...

18 October 2020 6,639 3 View

Probelm with expressing hetrologous proteins using Pichia pastoris?

07 December 2019 7,502 6 View

How to calculate flat membrane pore size distribution using membrane DI flux?

Almost all membrane pore characterizing techniques cannot be used due to the mechanical instability of the flat membrane. I want to use deionized water flux (DI) and mathematical way to determine...

04 December 2019 3,850 2 View

Which part of polypeptide is susceptible for reaction?

A given polypeptide protein has side chains and terminal (N- and C-). For any modification or conjugation reaction which part involved in reactions (side chains or terminals). Thank you !!

17 October 2019 575 2 View

For many days enzyme expriment, fresh medium of enzyme need to be replaced regularly. At what time interval the medium should be replaced ?

For many days enzyme experiment, fresh medium of enzyme need to be replaced regularly. At what time interval the medium should be replaced by new to keep activity of enzymes?

01 September 2019 7,619 4 View

Like the question: "To be or not to be", I cannot seem to go past wondering what is Ethics? & /or what is Morality?

What is Ethics? & /or what is Morality in Criminal Justice? Since crime can be a social, legal, or a violation of code(s), and sanctions "bestowed" in the name of "justice" may involve an...

01 May 2019 3,526 3 View

Can i used MTT to determine soil dehydrogenase?

In my understanding there are three main types of tetrazolium dyes that are used to determine dehydrogenase incluing MTT, INT, and TTC and for some reason i have encountered that MTT is rarely...

11 September 2018 3,590 2 View

Any idea why this cloning is not working?

Hi all, I am trying to clone a 750bp Age1/Xho1 fragment cut from a minipreped cDNA into another minipreped vector of 7.5Kbp (Amp) cut with the same enzymes. Insert and vector bands were cut and...

05 May 2018 9,946 12 View

What is the right journal to publish a survey paper in transportation?

What is the right journal to contact for publishing a survey paper in transportation?

05 March 2018 2,981 3 View

Oxygen Glucose Deprivation on mature oligodendrocytes cell lines not working?

27 December 2017 4,524 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How to prevent ANFIS Model from negative prediction values in MATLAB?

I have input and output data set for "ANFIS modeling in MATLAB", and I am getting some negative predicted values of output in testing. However, the predicted values of output in training are...

28 February 2021 3,459 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

No module named 'tensorflow.keras.backend.tensorflow_backend' ?

System Description Cuda 11.2 GeForce GTX 1080ti tensorflow version 2.4.1 keras version 2.2.0 python version 3.8 I was getting the below error when I try to run my code on the...

25 February 2021 6,673 2 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View