Hi Ehsan!

I worked with rat sciatic nerves before and FACSed cells derived from the nerve. It works well.

Do you need a protocol to stain and FACS cells from the cell culture or do you need also a protocol to digest the nerve and plate the cells?

Hi Levan , I need protocol for rat sciatic nerve not for cultured cells. 

I see. I suggest  you can try collagenase and dispase to digest the tissue, or papain or trypsin. After digestion you can try to triturate with glas pipette to have a single cell solution.