Has anyone did FACS analysis of Rat Sciatic Nerve and what protocol is used to analyse the monocytes?

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Levan Chikobava

Hi Ehsan!

I worked with rat sciatic nerves before and FACSed cells derived from the nerve. It works well.

Do you need a protocol to stain and FACS cells from the cell culture or do you need also a protocol to digest the nerve and plate the cells?

Ehsan Elahi

Hi Levan , I need protocol for rat sciatic nerve not for cultured cells.

I see. I suggest you can try collagenase and dispase to digest the tissue, or papain or trypsin. After digestion you can try to triturate with glas pipette to have a single cell solution.