Has anyone conducted PCR identification of Salmonella serogroups from animal sources, especially swine?

More Chinwe Iwu-Jaja's questions See All

After an error questionairee is discarded what happens next?

If a sample size is 300 and 5 Questionnaires are discarded due to error, are the 5 discarded questionairee not meant to be replaced? Just curious

16 April 2024 7,472 3 View

Environmental Agency data Vs Citizen Science Data on water quality?

Are there discrepancies between the environmental agency data on water quality and data gotten from Citizen Science?

03 May 2023 1,334 3 View

What can be use to mop up heavy metals from vegetables or soils in quarry mining site?

It has been established that quarry mining site polluted and contaminated it's environment with heavy metals, these affects the crops and vegetables planted in the area. The possible solutions to...

12 February 2021 1,565 5 View

Making vaccines using the virus's protein?

What is the process in using only parts of viruses such as proteins which can be recognized by the immune system to make vaccines such as in the case of the spike protein for Mers-CoV? I...

23 February 2020 1,297 5 View

What are the possible sources of error if i carried out Iodine staining on a TLC plate and no mark appeared?

I carried out thin layer chromatography and afterwards had to stain my plate with iodine as an alternative viewing method but the analytes did not take a brown or yellow color. The whole plate was...

20 February 2020 5,416 3 View

Comparing two chromatograms?

QUESTION I WAS ASKED: Compare the chromatograms in Appendix 1 and Appendix 2 and note your observations? MY QUESTION: What exactly am I meant to compare? I know you guys are not seeing the...

30 November 2019 3,711 2 View

What are the possible sources of error if I run a gel and can only see the DNA ladder under the UV light?

Hey guys, I did a restriction digest and ran my sample on an agarose gel. Under the UV light, all I can see are the bands of the ladder. A loading buffer was used.

29 November 2019 1,204 3 View

Is any one experienced in implementation research (IR)?

I am working on vaccines supply chain and I need assistance from someone who is experienced in IR. I am seeking for the best strategies to improve the supply chain and how to implement the...

16 February 2017 5,143 3 View

Do immunoglobulin A, G, M have any relevance in the "prevention from mother to child transmission of HIV" ?

Do immunoglobulin A, G, M have any relevance/importance/role in the "prevention from mother to child transmission of HIV"

06 July 2016 8,395 2 View

Can the immune system of HIV+ mothers determine if the child becomes HIV+ or not?

please i want an article that proved that the immune strength of HIV+ mother determines if the infant becomes HIV infected or not

05 July 2016 2,114 5 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,605 2 View

Best method for extracting RNA from morulae?

Best method and number of embryos for extracting RNA from pig morulae?

21 July 2024 4,398 1 View

Need a reference on pig residual analysis report and the parameters containing in it, for an oil and gas pipeline industry. Has anybody done it?

Pigging is basically a cleansing process followed in all pipeline industries after the product has been delivered. There are many types of pigs that are inserted inside the pipeline for this...

27 June 2024 1,221 1 View

How to add one of my recently published papers that has not been uploaded in the ResearchGate?

To whom it may concerns, One of my recently published papers has not been uploaded in the ResearchGate. Physiological Basis for Xenotransplantation from Genetically-Modified Pigs to Humans: A...

28 May 2024 8,172 2 View

What are local means to reduce pig death by natural diseases?

my pigs are dying what can i do please help researchers

07 May 2024 993 9 View

What is the breakpoint diameter for florfenicol and oxytetracycline in the disk diffusion?

What is the breakpoint diameter for florfenicol and oxytetracycline in the disk diffusion method, indicating susceptibility or resistance to these antibiotics in the tested salmonella strain?

25 April 2024 5,168 1 View

Please has anyone used Enrofloxacin with disc content of 10, Vancomycin (30) and Cefamezin (30) for disc diffusion method? What was the breakpoint?

I used these antibiotics, such as Enrofloxacin (10), Vancomycin (30), and Cefamezin (30), for Salmonella spp. as a disc diffusion method, and I'm finding it difficult to get the breakpoint for...

04 April 2024 2,710 0 View

Different species, same matrix, can I make different way to make STD and QC samples during method development and validation?

There days I was facing one challenge when doing MD and MV. I need to use rat and pig' plasma as matrix. I used to spike the solvent with different concentrations into the plasma to make STD and...

01 April 2024 3,766 0 View

Is possible to detected CD9, CD63, CD81 in grapefruit-derived EVs by WB?

I found one publication detected CD9, CD63, and CD81 via western blot. I feel a little confused about it. The antibody they used are specific for human, mouse (I checked the product in the...

20 March 2024 9,277 1 View

Where to determine nitrogen?

Maybe you can give me some recommendation on this matter. I have two reactors, one of them contains a solution of 90% water type 2 and 10% banana peels. On the other hand in reactor two there is a...

17 March 2024 4,408 1 View