I am looking for information on starch and protein gelatinization or denaturation during processing? We are working on the subject so we can share some results.
Dr. Ahmed
I am working in glucosinolate analysis and composition of different Eruca species.
As probably you know rocket leaf contains glucoraphanin, a glucosinolate with antioxidant and anticancer properties. I suppose glucosinolate content will be modified during processing. I wonder if you are also interested in the fate of rocket leaf glucosinolates. If so, we could collaborate in this research.
Thanks Antonio for your responses. Yes, the work you mentioned that is my next target. It would be great if we can collaborate in the work since you have expertize on glucosinolate and we have done other parts pertaining to processing.
From work done by O'Hare et al. a few years ago here in Australia, rocket's glucoraphanin content is about one-thirtieth that of broccoli. However, rocket is highest in glucoerucin, a precursor to erucin which is interconvertible with sulforaphane, the isothiocyanate for which there exists a large body of health-related research. So, why not assay for glucoerucin instead of glucoraphanin?
Thanks Christine. Do you have any method for the same as I am not a chemist so I can give the analysis work to other people who have HPLC
We have only assayed for Sulforaphane via HPLC but both Clarke and Mithen have published data on erucin and its quantification. I am not a chemist either; I'm a clinician.
Thanks Christine. I will go through the paper and do the needful with our HPLC team.
Glucoerucin and glucoraphin can be analysed by HPLC following standard methods as described in many papers.
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