The best of conjugation for peptide??? KLH or BSA?

hello which carrier is the best of conjugation for peptide or hapten? KLH or BSA? why? Thank you for your answers

30 October 2019 4,794 1 View

What could be the mathematical nature of production cost in supply chain networks?

In my research work, I want to construct mathematical programming model for a supply chain network problem. I have assumed production cost to be linear in nature. Is this assumption correct or...

15 July 2019 4,246 6 View

Let us start from easy questions:why to use chi square in quantitative analysis?

I think, the question does not need further explanation as the question is clear and concise, we have to explain why and where we use chi square while doing quantitative analysis?

14 January 2019 5,681 7 View

Hello,Can I evaluate Per1 gene expression in the blood of male rats?

gene expression

02 March 2018 6,365 1 View

My dear friends, What substance or combination do you recommend for enteric coating the Chitosan-Quercetin nanoparticles (pH 4-5) to colon delivery?

While the pH of my nanoparticle solution should not exceed 5. It should be noted that I only have a spray dryer device for this purpose. Thanks

30 December 2017 2,090 2 View

Which condition should be considered in Viral Nervous Necrosis (VNN) challenge?

One of my friends in Malaysia have tried 3 times challenged on their Grouper and Sea Bass fish with local NNV isolates, and all failed, meaning the fish did not die or show any clinical signs, but...

07 November 2017 9,093 2 View

What about relation of Microscopic damages of Viral Nervous Necrosis (VNN) in Brain and Retina in affected fish??

As you know vacoulation in CNS would be a typical lesion in VNN disease that was appeared more in adult affected fish.

31 October 2017 1,941 6 View

Solvation in c++?

Dear colleagues, I'm beginner in C++ programming, I've coded a c++ program to solvate (hydrate) a structure (carbon nanotube for instance). Would you please check it out and give me your comments...

03 February 2017 7,152 9 View

MPI error running LAMMPS?

20 November 2016 7,494 3 View

First report of Viral Nervous Necrosis (VNN), which time is correct?

Many scientists talked about first report of VNN in marine fish In many of scientific reports such as: 1) The first report of viral infection in Asian sea bass was made by Glazebrook et al....

25 August 2016 7,426 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View