How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Statistics: include groups of respondents made by one person for analysis?

Good morning, I could not find literature about this so I am asking. I am organizing data for a questionnaire (N=100) that foresees some personal questions like level of education. For the aim...

25 February 2021 2,090 4 View

What should a smart water system look like?

Our team is designing a smart water C&C dashboard for a smart city initiative. As we look into the current smart water system landscape, we couldn't reconcile to a definitive solution that...

16 February 2021 1,094 3 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Can anyone please suggest where I can find a reference for ReaxFF parameters for PTFE(Teflon) suitable for use in LAMMPS?

Trying to simulate a ice-PTFE interaction and am unable to find a reference which actually lists out forcefield parameters for PTFE-PTFE interactions using ReaxFF or even other force fields like...

08 February 2021 9,373 2 View

How do you incorporate a study that used non-parametric analysis into a meta-analysis where majority of studies used parametric?

I am calculating Hedge's g for effect sizes, so I am pulling means, SDs, and Ns, from each study. However, one study did a Mann-Whitney U test, and so reports Mean Rank, Sum of Ranks, U, Z, and r...

31 January 2021 1,182 5 View

Is there an open source tool the measures the GRAVY index of a peptide incorporating the PTMs and unusual amino acids (e.g. hydroxyproline)?

Right now, I am using ProtParam to compute GRAVY index of peptides. However, some of my peptides are post-translationally modified, and contains unusual amino acids. I assume that ProtParam does...

29 January 2021 8,300 4 View

Do i need to establish reliability in a quasi experimental study that seeks to employ a standardized instrument (WEAC & NECO past questions)?

I m carrying out a study on innovative teaching methods, the teaching methods are relatively new in the research area. however, teachers are to be trained on the application of these methods in...

24 January 2021 1,879 3 View

Which statistical test to use to see if there is a significant difference between the vigilance of bird species at different feeders (in SPSS)?

I have gathered data on bird species visiting 4 hanging feeders in different locations and have calculated the proportion of time they have spent displaying vigilant behaviour while at the feeder....

18 January 2021 9,959 7 View