Hallo, does any one know how to recover nitroxide labels on proteins for solid state nmr (MTSL on SH3)?

07 January 2016 1 3K Report

A few month ago I measured paramagnetic relaxation enhancement on MTSL labeled SH3 (solid state nmr). Now I want to go on with this topic by using the old sample again at first. But most probably the MTS radical degenerated. does somebody know an easy way to get the radical back ?

Tobias Schubeis

You dont get any PREs anymore? Maybe your radical label got reduced.. but that is usually only happening if you have compounds like ascorbic acid around. I am afraid that this rection is not reversible. Probably you will need a new sample...

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