Hello everyone. Recently, fueled by some friends' testimony on the difficulty of getting Pogonia ophioglossoides to germinate in-vitro, I began a few pilot experiments with the embryos to see if I could induce germination. There's a thesis in the literature that says this orchid can be germinated on K400 medium with peptone, but details about illumination and time to germination are omitted. I can't find the person's contact info either. In any case, I have seeds on K400 or BM2 (which contains peptone) with no additives, either coconut water or banana powder, and both additives, confounded by bleaching time trials. due to difficulty making the medium, i lost about half of my cultures to contamination (not too problematic right now as i am only doing a pilot study). something i noticed, however, is that the embryos are green! can they photosynthesize or react to light? in the thesis i read, a related species did better in the light, even though this is a terrestrial orchid. i put my cultures all under an LED so i could try to get germination. so far, 45-60 minute bleaching and then addition to coconut enriched medium (BM2 is better it seems, and banana makes no difference) has given the largest embryos, but nothing has erupted from a seed coat yet