I'm trying to extract an antimicrobial compound (active against Staph aureus) from the organic layer of a liquid-liquid extraction. What are some potential organic solvents I could resuspend the layer in that are not toxic to bacterial cells (whether in general or at a certain percentage)? I have already tried 5% DMSO and 5% isopropyl alcohol with little luck.
Hi Emily Laskey . You could try propylene glycol, glycerol or Ensolv.
Hello
You can also try ethyl acetate, propyl acetate and isopropyl acetate which shows no toxic effect at concentration lower than 2.5g/L.
Best.
You could try DMSO: I am using it for dissolving Nile Blue A, and it is fine for my Haloarchaea bacteria, at 0.002%-0.005%(v/v) final volume. Higher percentages are used for dissolving certain pharmacologically-active compounds, for injection into animals, where they cannot be dissolved in water or saline.
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