Glycoproteomics - FAQS.TIPS

More Maria Stella Ritorto's questions See All

Can anybody suggest an accurate multichannel pipette which I can use for a large scale proteomic approach (0.5-10uL )?

I am establishing an HTS method for protein quantitation and I need to work with more than 100 samples / day. I am using an 8-channel eppendorf multichannel, but I am struggling since mainly the...

07 August 2013 2,447 11 View

To remove BSA from cell medium

Hallo to everybody, I have a question about the possibility to remove BSA from the cell medium. Has anyone tried to do it by TCA/acetone precipitation? Are there any specific protocols or may I...

05 June 2010 6,819 6 View

No title

02 March 2010 6,606 3 View

polarized/non-polarized membranes

Hallo to everybody, I have a small question regarding plasma membrane in epithelia cells: it is known that they are high polarized (basolateral and apical membranes), but in case of cancer has...

01 February 2010 572 0 View

Enzyme activity assay

Hallo to everyone! I have a question concerning the measurement of enzyme activity in vivo. Does anyone know a simple a reliable method to study the differential activity of enzymes in serum?...

01 February 2010 4,668 11 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View

Dannel Wang

hi,

Someone in my lab also wants to do some work on the glycoprotein analysis recently, but money always seems to be a problem (MS here is sooo expensive>

Maria Stella Ritorto

Thanks Dannel!

Sure, I would like anyway to do it in the next weeks and maybe, as I said, using that kit (even I am not so crazy for kits, but I hope I can save time with it!).

By the way, thanks for the suggested book: I will check its avalaibility in our library otherwise I will search for an e-copy (do you know if it is present in the Web, as, for instance, GoogleBooks??)

Have a nice day and please keep in touch ;)

Cheers!!!

Hi Miguel! Thanks!

Yes, I am using already Coomassie and MALDI MS and I know as it works...My question was concerning all procedure, I mean, if anyone has already worked with that kit.

I had no so much outcome, that means, I guess I will enrich my complex sample (serum) with lectin coloums (ConA) and then load it on gels. Coomassie is easy and high MS-compatible and I will keep it for now....What do you think it is better as digestion mix for N-Glycans? (I have a mix and not a specific glycoprotein...)

Thanks in advance and have a nice day!

Cheers

Hi Miguel and sorry for the late reply....

Right, I will follow your method with PNGase-F, I guess is more appropriate for my aims.

Thanks for the helpful discussion about that and I will keep you and the group informed about my near-future esperince/experiments (and doubts... ;) ).

Have a nice and successful day!