How do you multiple impute item scores and perform a linear regression on factor scores?

I am developing a questionnaire and first performing an exploratory factor analysis. After I have the final factor structure, I plan on regressing the factor scores on some demographic covariates....

18 January 2021 883 8 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

28 August 2018 4,866 2 View

Why are my SYBR green standard curve dilutions not amplifying and have flat curves?

Please help! Recently our SYBR Green qPCR reaction using our housekeeping gene, RPLP0, and all the same conditions stopped working one day to the next. We isolate RNA from tissue culture human...

03 August 2017 2,418 13 View

How to extract enough RNA from jellyfish polyps (Aurelia aurita) to monitor the expression variations of some genes (RT-PCR and qPCR)?

I would be very grateful if the community could give me some tips: as these organisms are very small, I wonder how many polyps do I have to pool for one RNA extraction or if one is enough? What is...

29 January 2017 4,605 2 View

Does anyone know what the average cell diameter of Panc1 cells is?

27 November 2016 5,872 3 View

In case of infected root samples, is the infection stopped by the Gus solution or by the ethanol washing bath (+ ref please :) )?

In my case, I'm working with entire root systems of Medicago truncatula infected by an oomycete.

18 October 2016 2,471 4 View

Why can I not digest or sequence my plasmid?

I amplified a plasmid and now I have an eluted stock from a maxi prep i did. I sent the plasmid out for sequencing and it came back as no priming. I tried a restriction digestion with 2 different...

13 August 2015 4,170 6 View

Any suggestion on the seeding density for primary monocytes in a 96-well?

I am doing a one-day assay with primary monocytes and seeding them at 3*10^5/well, as per a colleague recommendation. However I see others recommend much lower, but they seem to have different...

03 June 2015 6,422 2 View

How do I stimulate cell cultures with beta-estradiol?

I have been doing cell culture experiments that involve treating the cells with estradiol (E2). There are many papers published that state these cells are responsive to estrogen. The problem is...

15 May 2015 2,953 6 View

I would like to identify a promoter for a gene. Given a super-scaffold containing the gene. How would I go about this and what tools are available?

Pax6 is known to have several promoters. I am interested in a promoter located upstream of the gene. The reference sequence for my organism is currently being assembled. I have a scaffold...

10 February 2015 267 3 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

Can I run spearman's correlation on non monotonic data? Is it advisable?

Hi all, I am running a partial correlation analysis on mood data. I want to see if there is a correlation between scores on a novel hypomanic mood scale and existing measures of hypomania - the...

25 February 2021 1,619 3 View

What kind of institutional environment do entrepreneurs need to achieve happiness?

Entrepreneurs are stressful, and happiness is a scarce resource. Happiness is crucial to business development.In addition to the work characteristics, individual characteristics have an effect on...

25 February 2021 876 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

Is it appropriate to use AIDA model (Assessment of Identity Development in Adolescence) on a non-clinical sample?

I am researching emotion regulation and identity development in adolescence. I've come across several inventories for assessing identity development and it seems like the AIDA scale is the most...

16 February 2021 1,261 1 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View