im using trizol to extract RNA from mesechymal cells using directzol coloumn. but the RIN is too low(1 or N/A). and earlier samples didnt have this problem.
Traceback (most recent call last): File "/home/aarushi/gromacs/cgenff_charmm2gmx_py3_nx2.py", line 54, in import networkx as nx File...
18 June 2024 4,888 4 View
Art x Mental Health Dear fellow researchers, Art has always been a way of expression for me. It has calmed my inner turbulence and helped me to make better decisions. Let's talk about how picking...
06 February 2024 2,654 1 View
I used Pfu polymerase for the PCR reaction I had set a month ago and I got a positive band. I tried repeating the experiment with the same parameters but I couldn't get any band. I have checked...
21 October 2022 5,141 3 View
using the CTAB method of DNA extraction, from the seeds of Mucuna pruriens (the velvet beans),the supernatant as well as the precipitate turns black every time. what could be the possible reason...
30 October 2019 7,590 0 View
Hello everyone! I am trying to use Optiprep density gradient centrifugation to get rid of cell debris from cell suspension of dissociated mouse intestinal myenteric plexus. Unlike suggested by the...
09 March 2017 7,628 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,537 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,618 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 806 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,365 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 223 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,418 1 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View