Genetic variation in bottle gourd?

More Vimbayi Chimonyo's questions See All

How can one determine permanent wilting point without thumb sucking published values?

Below is a protocol that I want to use to determine PWP. At the base of a 200 l drum, 8 holes are bored so as to allow drainage. The holes are covered with sieve clothes to prevent loss of soil....

24 October 2013 4,515 4 View

SWB model vs Aquacrop?

What is the major weakness of SWB model developed and modified by Pretoria university over AQUACROP?

29 September 2013 1,641 1 View

Can I add an irrigated duplicate as a treatment when quantifying crop water use?

Crop water use, which translates to crop evapotranspiration, is defined by FAO 56 as is the evapo-transpiration from disease-free, well-fertilized crops, grown in large fields, under optimum soil...

17 September 2013 8,923 6 View

What would be the best model to use to model water use within an inter-crop?

14 April 2013 2,832 1 View

In a pot experiment to measure transpiration rate of a crop at different water regimes, what is the best mulching material to minimize evaporation?

13 March 2013 1,591 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Otto Brinkkemper

Schlumbaum & Vandorpe (2012) published ancient-DNA research of Lagenaria in Vegetation History and Archaeobotany 21.They used three chloroplast primers. Details can be found in the attached PDF.

Jack Kornfield

Hi Vimbayi,

Please see:

http://tinyurl.com/zrgeey3

http://tinyurl.com/ja6xjof

http://tinyurl.com/zodsj7g

http://tinyurl.com/jus3o8d

Best regards,

Jack