The thing is rather weird. For one gene, I have 3 mutations individually tagged with mCitrine, driven by its native promoter, but when transformed into Arabidiosis, only one of which give fluorescence signal, while the other 2 not. When switched to another promoter, the same thing happened.Ever considering it may caused by the Agro strain we used(C58C1), but it doesn't make sense.

Any comments on it would be helpful.