I need a standard protocol for carrying out GC and GC-MS for root extracts for the quantification of contents like IAA. Which is a better method?
GC-FID will be more close to reality, however you must use standards in order to quantify. What is IAA?
The manuscript referenced below contains a protocol used for analysis of IAA and ABA derivatives via GCMS in NCI mode.
PILET AND SAUGY, Effect on Root Growth of Endogenous and Applied IAA and
ABA, Plant Physiol. (1987) 83, 33-38
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