Why DNA is isolated along with RNA extraction?

I am using CTAB method for RNA extraction. However, during RNA extraction DNA also isolated and DNase treatment lowers the quantity of RNA. Kindly let me know the reason why DNA is coming along...

23 December 2020 9,627 4 View

Need Data on Following Variables for Given Countries?

Variables: Money , Banking Assets, Banking Loans, Industrial Production Index, Supply Chain Managers Index Countries: Pakistan, India, Bangladesh, USA, UK, UAE, and China Time: As much as possible

25 November 2020 4,206 2 View

PhD Thesis Idea on Econometrics?

Would you please suggest PhD Thesis Idea on Econometrics with 3 Gaps.

23 February 2020 464 4 View

Arc shaped heat source process examples?

Hello everyone, I am looking for some examples in which line/arc-shaped heat sources can be used. For example, the laser sintering process used a moving point heat source. In a similar way, I am...

22 December 2019 8,820 2 View

Genomic sequence amplification???

I am uploading 2kb sequence files of three genes any expert can help me to analyse these sequences and designed suitable primers for me as i have tried so many times before but i failed to get 2kb...

20 September 2019 340 3 View

Genomic sequence amplification???

I want to amplify the 5 prime upstream sequence up to 2kb, of genes, after several attempts i did not got specific primers or some suitable amplifying condition to get that sequence. Please...

13 September 2019 4,806 4 View

How to calculate the sample size in muscle assessment studies?

We usually take the sample size from previous literature while working on muscle function assessment using some non-invasive technique like electromyography or mechanomyography. Any one knows...

06 February 2019 6,425 2 View

Calculation of anthocyanins in initial extracting sample?

I measure anthocyanin content in my samples. I measured the absorbance and put the values in equation. I am confused about the unit of of concentration. it should be mg/L? the initial...

05 September 2018 9,444 3 View

What are the superior properties which can only be seen/experienced in CNTs but not in Graphene, Graphene oxide or reduced graphene oxide?

Since graphene discovered (2004) later then CNTs (1991) it surpassed the CNTs in respect of many properties like adsorption and chemical catalysis due to high surface area available in case of...

07 August 2018 8,702 5 View

How to do one way ANOVA when one sample is larger than the other?

I want to compare the means of teachers and students perceptions collected from two likert scale questionnaires. I have 112 teacher respondents and 60 student respondents. Should I use all the...

26 February 2018 192 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to design a boost converter for a mppt controller?

I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...

28 February 2021 3,637 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View