Trichoderma reesei is the hyper cellulolytic fungi, which is used for commercial cellulase production. There are many strains, which are used in labs for cellulase production, e.g T. reesei Rut C-30. According to your choice of fermentation condition( solid state or submerged), media composition, temperature, initial pH and many other culture conditions, the best substrate has to be find out and optimized. To my knowledge, cheap cellulosic materials, such as sugar cane bagasse, saw dust, paper waste, wheat straw, rice husk etc., are preferable. Gud luck :)

thanks for your answer....would you please give me some reference for operation condition the production of cellulase....i did the some experiment, but my cellulase enzyme is still low and sometimes contaminant with other microbes...

and also how to increase the activity of enzyme cellulase? example in the first production i just got 2 FPU (filter paper unit) or IU.... how to increase to be 40 FPU...?

Dear Muri, you have used only one strain of Trichoderma ressei or have screened many strains. You first go through screening process with optimizing all process parameters like substrate composition, ph and temp. You may use placket burman design for substrate composition optimization. You have not mentioned about down stream processing of your enzyme. Try to got through microfiltration followed by Ultrafiltration process, you may get better enzyme yield.

hi Muri, some people would have got upto upto10 FPU, but they d ve used solid state fermentation. First, select your mode of fermentation, whether it is going to be solid state or submerged???? my advice is to used solid state . Next, what growth substrate you are going to use? my advice is to use some cost effective cellulosic wastes. next, what pH and temperature,,, for this, you have to stream in the range pH 4-6 ,,, 27 deg to 32 deg. instead of going this one by one you can use some statistical tools, as Ranveer said, either placket burman ( to get an idea about the influence of each and every medium components on the production of enzyme) or response surface methodology ( to get the knowledge about the interactions of culture conditions with each other).

moreover, getting 40 FPU in the crude sample is merely impossible. you carefully read the paper, they may be concentrated one.....

so, my answer is to get better production, optimize, optimize and optimize the conditions and screen for the best growth susbstrate. Gud luck :)