You can try this protocol. It is easy to handle

http://jcm.asm.org/content/38/1/471.full

best regards

Ruhi its having mercaptoethanol.. tris hcl, edta, c-tab.. n i crush by liq n2... although it worked for macrophomina bt not for aspergillus

You can try this protocol. It is easy to handle

http://jcm.asm.org/content/38/1/471.full

best regards

@ruhi: i did with liq n2 only once but no diff

@sbissi; i wl try it

I have a good protocol for DNA isolation from Aspergillus nidulans, I can send you if you want but it's in Spanish :S.

I m sorry.i dnt follow spanish... Cn i hv a translated version

Hello every1.. I was wrking with aspergillus... N the buffer i was using did not hv sds ... So i made anw buffer with sds n it wrked.... @ laksmi... Mercapto conc in my lysis buffer is low