From where I can do HPAEC - PAD analysis in India?

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Why does the community engagement strategy not get priority in NCD prevention and control in developing countries?

NCDs are increasing alarmingly even in developing countries. Need to take participatory policies for prevent and control of NCDs.

30 July 2024 7,942 3 View

GC-MS calibrated for 16 EPA PAHs. Last 3 compounds show poor linearity. Response decreases. How to improve?

I have calibrated my GC-MS for 16 EPA PAHs using available methods. However, the last three compounds (Indeno[1,2,3-c,d]pyrene, Dibenzo[a,h]anthracene, and Benzo[g,h,i,]perylene) are not showing...

13 May 2024 4,966 2 View

How to Convert µg/mL standards to ng/m³ for PAH analysis in air via GC-MS?

Researchers typically use micrograms per milliliter (µg/mL) standards to calibrate gas chromatography-mass spectrometry (GC-MS) instruments for the analysis of polycyclic aromatic hydrocarbons...

12 May 2024 6,259 5 View

While doing propeller transient simulation on Ansys fluent, rotating zone contours corrupted. What is that reason?

I'm doing 3000rpm drone propeller computation on ansys fluent. First 1500 iteration steady state mrf zone is computed with SST k-w turbulance model. After convergence is done i change it to...

24 February 2024 9,935 1 View

How to calculate polydispersion index of a nanoparticle size? and does a PDI of 0.5 consider acceptable?

PDI value

30 November 2023 7,099 3 View

Is there an alternative chemical to ethylene glycol for IHC cryopreservation solution?

I am trying to optimize my antibodies for free floating IHC-IF on paraformaldehyde fixed mouse brain sections. I want to preserve my slices in an appropriate cryopreservation solution for later...

19 September 2023 6,240 3 View

How can we develop more secure and efficient encryption algorithms to protect data from cyberattacks?

a standard encryption method that could be used to protect the data since all data are mostly uploaded to cloud now a days , also cybercriminals constantly developing new ways to break...

17 September 2023 2,934 5 View

Does a Post Doctoral from AACSSB accredited institute has value?

I would like to know about the future career prospects for a candidate pursuing Post Doctoral from an AACSSB accredited institute

16 August 2023 5,508 2 View

How can I detach my mesenchymal stem cells from flask?

Hello everyone. About three months ago, I could detach my mesenchymal stem cells obtained from 8-week-old mice with trypsin %0.25 on the 10th day in 3-4 minutes. However, now I can not detach the...

03 May 2023 6,159 5 View

Does anyone having this book as PDF format?

THE ZEBRAFISH BOOK A guide for the laboratory use of zebrafish Danio* (Brachydanio) rerio by Westerfield, Monte

20 February 2023 5,940 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View