The Nusselt correlations for the high aspect ratio and high Rayleigh number?

I want to calculate natural convection in the horizontal rectangular enclosure (heated from below) and in the vertical rectangular enclosure by using the Nusselt correlation. However, I can not...

25 April 2020 648 9 View

qRT-PCR melting curves are good for cDNA of overexpression cells but not for cDNA of parental cells. What could be the reason?

I am trying to find a good qRT-PCR primer set for a gene that has low expression in our cell lines. As you see in the attachment I have a very sharp one peak when I used cDNA from overexpression...

23 February 2020 2,474 5 View

Viscosty effects in 1H NMR measurements?

I would like to see the derivatization of my polymer by 1H NMR. However, I cant see any peak. My sample becomes viscous in water (in the case of NMR it is in D2O). I did a similar test before and...

22 August 2019 5,020 3 View

What kind of skills, different from ordinary entrepreneurs, innovative entrepreneurs must have in terms of successful marketing?

With the new technological era, ordinary entrepreneurs leave their places to innovative entrepreneurs and the old way of marketing has also started to change. Innovative entrepreneurs must have...

02 March 2018 1,908 8 View

Anyone encountered this "Interesting" signal in LC/MS runs yet: 321.16 m/z?

It is (1+) and strongly present with high ACN content at the end of our LC gradients (Setup: Ultimate 3000RSLCnano HPLC coupled to an impactII MS running plant proteomics samples). Didn't find any...

09 March 2016 5,155 6 View

How to fix unstable GUVs with particles/tubes inside?

After electroformation, our unilamellar vesicles act very dynamic and unstable on BSA passivated slides (especially as their sizes get bigger). We sometimes observe them bursting and reforming....

07 February 2016 2,858 4 View

Could CGRP antagonists be helpful in the fight against COVID-19?

Can CGRP antagonists, which are seen as a new treatment option in migraine treatment, be a treatment option in COVID-19. What is your opinion? I share a study on this

01 January 1970 9,487 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Concentration of DNA?

Hello everyone! Is a normal value 295.8 ug/ul for cDNA? If not what does it mean? Where is the mistake? The measurement was done with nano drop. Thank you!

09 February 2021 6,202 1 View

What is the reference value of THD in BLDC MOTOR speed control ?

i used to control EHO+ANFIS to MRPID to Landsman converter for bldc drive i got 7% but some of them are telling IEEE standards are < 5% is it correct for Drives ?

07 February 2021 5,892 1 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

How to know the threshold limit value of a sar image...??

i want to do thresholding by segregation of water areas from non water areas for that i am using model maker in ERDAS but i am unable to get the threshold limit value of my image.

26 January 2021 8,277 1 View

Can anyone help to find any research articles related to the below mentioned objective?

Astaxanthin used in surimi based products as colour enhancer or as an antioxidant! Regards & Thanks Asik Ikbal

25 January 2021 9,399 4 View

How could i explain the EC50 data utilized by graph Pad prism 9 ?

I measured the FARP antioxidant activity of plant extract than i used graph pad prism 9 to calculate the EC50, now i am not sure how to describe these data as i got the logECF and ECF .

17 January 2021 4,576 3 View

No title

14 January 2021 8,086 37 View