Hi every one
How can I determination Formaldehyde in Shampoo by HPLC or LC-MSMS?
Why? HPLC is like using a 'shotgun to kill a fly'. Try using a 'fly swatter' instead. Many labs use malonic acid procedure and measure the corresponding yellow colour on a spectrophotometer at 420 nm.
depends on the sensitivity You need. I guess in reality purge and trap GC-MS is the best choice as formaldehyde has too low mass to work fine on LC-MS
The way to do an effective job with HPLC or LC/MS/MS is to derivatize the formaldehyde (and other small chain aldehydes) with a reagent like DNPH (dinitrophenyl hydrazine). This has the advantages of 1) attaching a chromophore so that spectroscopic techniques can be used for detection, 2) it increases the mass of the analyte so that LC/MS/MS can now be useful and 3) allows for chromatographic separation from sample matrix and other aldehydes, which then complements your detection choice (spectroscopy or mass spec)
As Charles states, most methods use derivatization. Many carbonyl compounds in the sample may form derivatives. Hopefully the HPLC will separate them.
Thanks Charles for your attention and advice.
Will you introduce exact method for determination of derivatized formaldehyde. For example introduce article.....
Thanks Bruce for your answer.
Could you more describe about this procedure!
Also Thanks Kai for your recommendation.
But we dont have GC-MS.
We need a method that we can validate in our laboratory with satisfactory accuracy and precision. Also, it must be Quick and easy.
Mahsa - there is a US EPA method called SW-846, Method 8315 that is designed for analysis of carbonyl compounds in water, soil/solids and wastes. The derivation process/chemistry is described in that method.
If quick and easy is a must for you, then the suggestion of headspace GC/MS may be the best choice for you as the derivatization followed by LC/MS/MS will be a little more involved.
- Badges
- Science topic