Hi everyone,
I generated a stable cell line with Flag-tagged protein, Flag is in N-terminal.
While the signal of the protein is very strong by WB and the size is also correct, but there is no signal when I blot for Flag. The positive control (transient transfection of this plasmid in 293T) is fine, so the antibody works.
So anyone can help to figure this out? How can I detect the fused protein with anti-Flag antibody?
Thanks!