23 December 2022 1 4K Report

Hi everyone,

I generated a stable cell line with Flag-tagged protein, Flag is in N-terminal.

While the signal of the protein is very strong by WB and the size is also correct, but there is no signal when I blot for Flag. The positive control (transient transfection of this plasmid in 293T) is fine, so the antibody works.

So anyone can help to figure this out? How can I detect the fused protein with anti-Flag antibody?

Thanks!

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