Dear Researchers,
I need a comprehensive guideline to follow to determine analysis cut-off points (e.g.: number of nuclei per sample, percentages to consider a deletion present, false +/- testing for each probe since the % of hybridisation is different for each probe).
I am using Leica: TP53, LEU1, +12 and ATM mutations for my FISH experiments. So far the only one I have found that is of any use is this:Article Guidance for Fluorescence in Situ Hybridization Testing in H...
Does anyone have any other papers that may be useful/helpful for my purpose? Would be better if it was specific to CLL.
Thanks in advance.
Hi.
The "AGT manual for cytogenetics" has a chapter on cytogenetics mathematics, including cut-off calculations. Hope it might help you.
Hi dear Darellynn Oo
I think the following link can help you;
Best and kind regard.
https://www.onclive.com/publications/oncology-live/2011/december-2011/fish-testing-guides-treatment-of-cll-new-drugs-on-horizon
Hi Mehdi,
It looks like a good book. I saw some interesting chapters that may be of use but I tried everything from the university library to online access and even with the institutional login, I am unable to download it. Do you happen to have a copy?
Sure! That's a great book. I have a hard copy of it but I can email you some pictures from those pages which are about cut-offs. Please send me an email, then I can attach them in reply.
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