8 µg (in 1.6 µL) of 6-OHDA was introduced into the right striatum of mice according to the coordinates A = 0.5, L = 2.0, and V = -3.5 relative to bregma. The needle was withdrawn 5 min after the injection. Seven days after operation, 0.5 mg / kg apomorphine was used to induce rotation, Unfortunately, only a few mice were able to induce seven rotations per minute. Can someone please tell me what went wrong? How can I improve the surgical or experimental protocol？
David Hubbard
Did you verify surgical location? You should verify canula placement prior to behavioral testing. Then again after the experiment, you need to verify placement and the effectiveness of the lesion. Stain for a marker of dopaminergic phenotype.
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