Extrusion spheronization method

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How does a pH 13 buffer solution containing NaOH/KCl work?

pH 13 Buffer solution is prepared from a strong base sodium hydroxide and potassium chloride salt. However a buffer contain a weak base or weak acid with their salts. What is the purpose of the...

26 January 2021 9,386 1 View

Can we identify the minerals of red soil using these XRD data????

I need to identify the minerals in red soil because I want to separate the irons from that. so that purpose I want to know the other minerals contain in the red soil. then only I can remove the...

28 September 2020 1,208 9 View

Does anyone have supportive document related to thermodynamic modeling tools such phreeqc/GEM ?

I need to do some calculation works related to concrete using thermodynamic software. If anyone has supportive documents, please help me.

09 July 2020 6,976 2 View

Solubility of a film

what are the possible ways and instrument techniques to determine the solubility of the artificial film which could be coated?

01 January 1970 7,892 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

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How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

Why my plant protein cannot resolubilize after TCA/Acetone extraction?

I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...

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Which size of undersized iron pellet is suitable for sponge iron tunnel kiln production?

Someone advise me to use 1 to 2mm undersized pellet but it is not available. Do anyone have any experience in the field of tunnel kiln ?

28 June 2024 6,355 0 View

How to properly label fish feed pellets with stable isotopes for tracing C and N flow?

Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...

19 June 2024 746 0 View

Can untransformed pET22b be isolated from five days old pellets stored at -20°C?

I've pelleted down the overnight ampicillin supplemented cultures of DH5ALPHA for pET22b isolation 5 days ago and stored them on -20°C without adding any buffer or glycerol. Would the cells be...

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