For detecting enkephalins in mouse brain samples by ELISA, finding a well-validated kit can be challenging due to the small size and similarity of opioid peptides.

Some researchers recommend looking for ELISA kits that specifically target [Met]enkephalin or [Leu]enkephalin and checking their validation data for brain tissue. Vendors like Phoenix Pharmaceuticals and Abcam offer enkephalin ELISA kits that have been used in neuroscience studies, but I suggest carefully reviewing product datasheets and published references.

Alternatively, if ELISA kits are limited, radioimmunoassay (RIA) or mass spectrometry methods may provide more sensitivity and specificity for brain enkephalins.

If you want, I can share some references and suppliers I’ve come across in my research on neuropeptides