Chicken egg yolk is widely used in the cryopreservation experiment of mammalian sperm, especially bull, as a cryoprotectant. Regarding that, I read several publications that use egg yolk as a cryoprotectant on cryopreservation of chicken sperm. Given that egg yolk in chicken is homologous to oocytes in mammals, could this method be justified? Aren't the sperm will undergo a premature acrosomal reaction when mixed with egg yolk?
Robert Renema
Avian sperm would not normally interact with egg yolk in the form you are referring to. To get to the site of fertilization, they must get through the perivitelline membrane to the germinal disk. The yellow yolk material lies beneath the germinal disk, so the sperm will not come into direct contact with it.
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