Temperature and disinfectants both play crucial roles in affecting bacterial growth.Temperature:High Temperatures: Most bacteria have specific temperature ranges for optimal growth. High temperatures can denature proteins and disrupt cell structures, inhibiting bacterial growth. However, extreme heat can kill bacteria outright.Low Temperatures: Cold temperatures can slow down bacterial metabolism, preserving food and inhibiting growth. Some bacteria can survive in a dormant state in cold conditions.Disinfectants:Chemical Agents: Disinfectants, such as bleach or alcohol, can disrupt bacterial cell membranes, proteins, and nucleic acids, leading to cell death.Concentration and Exposure Time: The effectiveness of a disinfectant depends on its concentration and the duration of exposure. Higher concentrations and longer exposure times generally result in better bacterial eradication.
37C will not be optimal for many environmental microbes, 65C will eliminate many (Pasteur established 60C). Wonder at agar gel stability but it will certainly dry out.
At 100C you won't have an agar gel, few microbes will survive and very few will grow.