You can find your answer in the following publication : A unified procedure for detecting, quantifying, and validating electrocardiogram T-wave alternans

If by beats you mean finding the time of the R-spike/QRS-complex, I evaluated some algorithms for R-peak detection lately. The following, with some tweaks, ended up being most robust of the few and relatively simple to implement. http://link.springer.com/article/10.1007/s13239-011-0065-3/fulltext.html

I can publish an implementation in Python if that would be of help.

Edit: Right article.

Dear Pekkanen,

Thank you for reply.

My first question is: Which ECG segmentation method is recommended? in some papers it is reported that we should first detect the R peak and then choose 100 samples after and 100 samples before the R peak as a segment. some other have chose 150 samples after the R peak as a segment. Which method do you prefer?

Second question: If i want to segment the ECG into typical beats (include P-Q-R-S-T waves), what should i do? Should i choose the T(offset)-T(onset) interval as a beat? I can detect the R peaks,but i have problem on other peaks. Which method do you recommend?

I would appreciate if someone help.

It seems gave a wrong article in my previous post. I was actually referring to this method: http://link.springer.com/article/10.1007/s13239-011-0065-3/fulltext.html

I haven't really done further processing than finding the R-peaks and calculating RR-intervals for studying arrhythmia. Unless there's need for very very very high accuracy (

The implementation I mentioned can be now found here: https://github.com/tru-hy/rpeakdetect

Dear Akbar,

I can only answer the first question, since I only have experience with ECG beat segmentation, not with detecting other specific waves.

The segmentation depends on the sampling frequency and heart rate. For a sampling frequency of 250 Hz and normal heart rate of about 75 beats/min, you can take 100 samples before and 100 samples after the R peak. This is because 100 samples = 0.4 s. So, 200 samples = 0.8 s, which is a normal size of one heart cycle for 75 beats/min rhythm.

The method of segmentation also depends on what you are analyzing. If the QRS complex is the most relevant (for example, for studying PVCs), the proposed segmentation method should be the best. However, if the focus is on T-waves or P-waves, it may not be the most appropriate.

You could epoch the ECG time series data with R-peak as the trigger point, pre and post triggers with ref to it.

Identify R-peak using some standard algorithm and accordingly fix time limits to include values upto P-wave in the front and T wave at the back.

The conventional trigger based epoching approach in EEG experiments is the inspiration.

You can use the Pan-Tompkins algorithm or Wavelet transform to detect QRS wave from there you can detect R peak.

After detecting QRS by using filtering or basis expansion or thresholding you can detect p and t wave of ECG.

Or by using Extended kalman filter and dynamic model of ECG you can predict p,q,R,S,T wave.

Hi Akbar, I am doing research in this area and University of Rochester hosts the worldwide THEW (Telemetric and Holter ECG Warehouse):

http://thew-project.org/

Please register with the site (completely free, an NIH sponsored project). Once you access the information, there are algorithms and a wealth of data there ...

But, just to answer your question, the pre-processing steps are:

*** R peak detection

*** Detection of QRS boundary

*** End of T wave detection

============================

R Peak Detection includes:

*** Noise reduction, Lead selection, Extraction of QRS template, R peak detection, removing false-detected T waves

============================

QRS boundary detection uses an algorithm by W Zong (MIT).

There is a lot more detail, but, this should help get you started I hope ...

P-P.

On segmentation the following paper might be useful:

JL Salinet Jr, JPV Madeiro, PC Cortez, PJ Stafford, G Andre Ng, FS Schlindwein, “Analysis of QRS-T Subtraction in Unipolar Atrial Fibrillation Electrograms”, Medical & Biological Engineering & Computing, ISSN: 0140-0118, IF: 1.878, DOI : 10.1007/s11517-013-1071-4, 26 March 2013.

Of course the classical Pan-Tompkins detector is always a very good starting point too.

It also depends on how R peaks are initially detected. As many suggested other ways to detect R-peaks. I used extrema function and applied threshold to detect the R-peaks. Now, In the same array of extrema, I also get PQRS points. The number of samples for all points (PQRST) is within +/- 30 maximum (with reference to R-peak) (in my case) . In any method of R detection, the number of samples between 'P' to successive 'P' need to be considered.

For Rpeak and Tend detection use algorithm stated in this paper..very good sensitivity and specificity.

Dear you can also download code for PQRST waves detection http://www.mathworks.com/matlabcentral/fileexchange/45404-ecg-q-r-s-wave-online-detector/content/peakdetect.m

I am working on T wave extraction 

First and foremost step in epoching ECG time trace beat-by-beat is to identify the R wave peak instants, then include few milli seconds of data prior (pre-trigger)to R wave instant to accomodate the P wave, then a few milli seconds after the R wave as (post trigger) to include T wave. Repeat it for every R wave instants. You might note that the Pre and post trigger would significantly vary across subjects, or for the same subject on different occasions  due to the fact that heart rate will influence the cardiac intervals like PR and QT intervals. 

I suggest you use Empirical Mode Decomposition (EMD) to filter noisy parts and estimate background signal of ECG signals. Visit here for real ECG data and Matlab codes. 

https://onsignalandimageprocessing.blogspot.com.tr/2017/03/qrs-detection-algorithm-using-empirical.html