http://www.nature.com/articles/srep23743/figures/2

view this paper it shows the conservation level of ebola protein , may help.

Hello Mohammad and thank you for your kind and informative reply.  I have read this article and still think that the most conservative site is still the receptor binding site of GP1.

I wish to model and simulate a nanorobotic sensor able to detect Ebola in vivo during its low count nonsymptomatic stage, accordingly am in search of an extremely conservative region that is recognizable and not changing/mutating.  

The problem with the receptor binding site on GP1 is it is naturally buried, suggestions?

Best,

Joel

Dear Joel

Pls. find the attached files and the references, good luck

Hoping this will be helpful to your project

Regards

Prof. Houda Kawas

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406178/

https://pharmaceuticalintelligence.com/tag/drug-resistance/

https://worldwidescience.org/topicpages/d/dna+hybridization+assays.html

http://www.transterrestrial.com/archives/technology-and-society/

http://www.rki-i.com/Vortrag%20Rheuma/5560a47f7498b7c40c7ccbb02891c3ed8401d36c.1.pdf

http://www.academia.edu/20195680/Microbes_Concepts_and_Applications

http://gprcp.ac.in/seminar/Abstract%20book%20Final%202015-%2031-7-15.pdf

https://uglesiden.wikispaces.com/nyhederudvalgte2017februar

Also

http://www.ida.liu.se/~petbu/conferences/biomedsim2005/ProceedingsBIOMEDSIM2005.pdf

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&cad=rja&uact=8&ved=0ahUKEwib2M-tnIPTAhVJOpoKHbLzCW0QFgg3MAQ&url=https%3A%2F%2Fwww.researchgate.net%2Fprofile%2FPrakash_Bisen%2Fpublication%2F229088964_Microbes_Concepts_and_Applications%2Flinks%2F00b49533994a00a6aa000000%2FMicrobes-Concepts-and-Applications.pdf&usg=AFQjCNH0zxU3-fYqoFtAsbYCjASy1fxklQ&bvm=bv.151325232,d.bGs

Hello Houda and much thanks for your response and great information.

I scanned all of the above articles and still can not find my very particular answer to my question of which is the most highly conserved region in the Ebola genome (I believe its the receptor binding protein of the GP1).  If you gain insight to this answer feel free to reply, thanks a bunch:)

Best,

Joel

Dear Joel

It's not my project, but

GP one of it, glycosylation sites and experimentally determined epitopes, can identify the most promising regions.

Pls. find attached files with the references

Hoping this will be helpful to your project, good luck

Regards

Prof. Houda Kawas

http://www.sciencedirect.com/science/article/pii/016817029390063S

https://academic.oup.com/bioinformatics/article/33/2/151/2525716/Evolutionary-conservation-of-Ebola-virus-proteins

http://www.cell.com/cell/abstract/S0092-8674(00)81248-9?_returnURL=http%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0092867400812489%3Fshowall%3Dtrue

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529428/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5090869/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848411/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551310/

https://genome.ucsc.edu/ebolaPortal/

https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-8-44

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551310/figure/fig1g/

Also

Hoping this will be helpful

 Hello Houda and thank you again kindly for this more related information.

Yes this L-Protein is dilute but present in Ebola infected patients during the first week non-symptomatic stage and may be a good target for a nanorobotic sensor in vivo due to its highly conserved regions, further investigation is needed:)

Best,

Joel

You most welcome

Yes, this is true

Houda

Aloha,

if you have time please scan my subsequent question posted entitled:

'How dilute is Ebola viral trash floating around the blood stream when first infected?'  Since this addresses sensing this trash via a nanorobotic in vivo before symptoms begins.

Best,

Joel

Dear Joel

I will answer you soon

The problem is known for early detection of most viruses

The problem in the presence of electricity to work on the computer

Houda

Hello and ok I will wait for your answer?:)

Joel