Dear Sir. Concerning your issue about whether the EPS extraction method from biofilm affect its viscoelastic proprities. The microorganisms in biofilms live in a self-produced matrix of hydrated extracellular polymeric substances (EPS) that form their immediate environment. EPS are mainly polysaccharides, proteins, nucleic acids and lipids; they provide the mechanical stability of biofilms, mediate their adhesion to surfaces and form a cohesive, three-dimensional polymer network that interconnects and transiently immobilizes biofilm cells. In addition, the biofilm matrix acts as an external digestive system by keeping extracellular enzymes close to the cells, enabling them to metabolize dissolved, colloidal and solid biopolymers. Here we describe the functions, properties and constituents of the EPS matrix that make biofilms the most successful forms of life on earth. EPS retain on the membrane as a result of the layer viscoelastic properties. These results highlight an important relation between washing efficiency of fouling layers from membranes and their viscoelastic properties, in addition to their adhesion properties. I think the following below links may help you in your analysis:
http://www.nature.com/nrmicro/journal/v8/n9/full/nrmicro2415.html
http://pubs.acs.org/doi/abs/10.1021/acs.est.6b01458?journalCode=esthag
http://www.rug.nl/research/portal/files/6651465/Thesis_e_version.pdf
Thanks
Dear Isam,
i am aware of what is EPS and its proprieties, however i am interested in whether EPS extraction will affect its proprieties compared to the version (not extracted) EPS in the biofilm.
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